Alcohol-Perturbed Self-Assembly of Tobacco Mosaic Virus Coat Protein

  1. Ismael Abu-BakerORCID Logo and
  2. Amy Szuchmacher Blum

Submitting author affiliation: McGill University, Montreal, Canada

Beilstein Arch. 2022, 20222. https://doi.org/10.3762/bxiv.2022.2.v1

Published 05 Jan 2022

  • Preprint

Abstract

Self-assembly of Tobacco mosaic virus coat protein is significantly altered in alcohol-water mixtures. Alcohol cosolvents stabilize the disk aggregate and prevent formation of helical rods at low pH. High alcohol content favours stacked disk assemblies and large rafts, while low alcohol concentration favours individual disks and short stacks. These effects appear to be caused by the hydrophobicity of the alcohol additive, with isopropyl alcohol having the strongest effect, and methanol the weakest. We hypothesize that alcohols interact with the hydrophobic faces of TMV-cp disks, thereby disrupting the protein-protein interactions between disks that are necessary to form helical rods.

Keywords:

Supporting Information

Format: DOCX Size: 10.3 MB   Download

How to Cite

When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Abu-Baker, I.; Blum, A. S. Beilstein Arch. 2022, 20222. doi:10.3762/bxiv.2022.2.v1

Download Citation
Download RIS (Reference Manager) Download BIB (BIBTEX)

© 2022 Abu-Baker and Blum; licensee Beilstein-Institut.
This is an open access work licensed under the terms of the Beilstein-Institut Open Access License Agreement (https://www.beilstein-archives.org/xiv/terms), which is identical to the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0). The reuse of material under this license requires that the author(s), source and license are credited. Third-party material in this work could be subject to other licenses (typically indicated in the credit line), and in this case, users are required to obtain permission from the license holder to reuse the material.

Other Beilstein-Institut Open Science Activities

Logo
Logo
Logo
Symposia