The interaction between cucurbituril (Q) and chloramphenicol (CPE) was investigated using single-crystal X-ray diffraction, spectroscopy, isothermal titration calorimetry (ITC) and UV-Vis, NMR and IR spectroscopy. The effects of Q on the stability, in vitro release performance and antibacterial activity of CPE were also studied. The results showed that CPE and Q formed a 1:1 inclusion complex (CPE@Q) with an inclusion constant of 5.4736 × 105 L/mol. The intervention of Q did not affect the stability of CPE, but obviously reduced the release rate of CPE in artificial gastric and intestinal juice; Q has a slow-release effect on CPE. The antibacterial results showed that the minimum inhibitory concentration (MIC) of CPE and CPE@Q toward Escherichia coli (E. coli) was 1.5 × 10–3 and 1.0 × 10–3 mol/L, respectively, and toward Staphylococcus aureus (SA), the MIC was 2.0 × 10–3 mol/L for both CPE and CPE@Q. Therefore, Q enhanced the inhibitory activity of CPE against E. coli.
Keywords: Chloramphenicol, cucurbituril, host-guest interaction, stability, in vitro cumulative release, antibacterial activity.
|Format: DOCX||Size: 26.6 KB||Download|
When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:
Zhang, L.; Zheng, J.; Luo, G.; Li, X.; Zhang, Y.; Tao, Z.; Zhang, Q. Beilstein Arch. 2021, 202162. doi:10.3762/bxiv.2021.62.v1
|Download RIS (Reference Manager)||Download BIB (BIBTEX)|