A high yield gold nanoparticle-based DNA isolation method for human papillomaviruses genotypes from cervical cancer tissue samples

  1. Noorossadat SeyyediORCID Logo,
  2. Fatemeh Farjadian,
  3. Ali Farhadi,
  4. Gholamreza Rafiei Dehbidi,
  5. Reza Ranjbaran,
  6. Farahnaz Zare,
  7. Mohammad Ali Okhovat,
  8. Negin Nikouyan and
  9. Abbas Behzad-BehbahaniORCID Logo

Submitting author affiliation: Shiraz University of Medical Sciences, Shiraz, Iran, Islamic Republic of

Beilstein Arch. 2019, 2019106. doi:10.3762/bxiv.2019.106.v1

Published 16 Sep 2019

  • Preprint

Abstract

Gold nanoparticles (AuNPs) are commonly used in biosensors of various kinds. The purification of DNA from cancer tissues is an important step in diagnostic and therapeutic development, but current methods are not optimal. Many cervical cancer patients are also susceptible to high-risk human papillomavirus (HR-HPV) infection. Accurate viral diagnosis has so far relied on the extraction of adequate amounts of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. Since the sensitivity and specificity of commercially available purification kits are not optimal, we designed a DNA purification method based on AuNPs to purify sufficient amounts of HR-HPV DNA from cervical cancer tissue samples. AuNPs were coated with a series of oligonucleotide probes to hybridize to specific DNA sequences of HR-HPV genotypes. With this method, we recovered 733 out of 800 copies of type-specific HPV DNA with complete specificity, compared to 36 copies with a standard commercial kit (Qiagen FFPE).

Keywords: Biosensor; Cervical cancer; Gold nanoparticle; Human papillomavirus; Oligonucleotide probe; Paraffin embedding

Supporting Information

Format: DOCX Size: 484.5 KB Download

How to Cite

When a peer-reviewed version of this preprint is available in the Beilstein Journals, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Seyyedi, N.; Farjadian, F.; Farhadi, A.; Rafiei Dehbidi, G.; Ranjbaran, R.; Zare, F.; Okhovat, M. A.; Nikouyan, N.; Behzad-Behbahani, A. Beilstein Arch. 2019, 2019106. doi:10.3762/bxiv.2019.106.v1

Download Citation
Download RIS (Reference Manager) Download BIB (BIBTEX)

© 2019 Seyyedi et al.; licensee Beilstein-Institut.
This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0). Please note that the reuse, redistribution and reproduction in particular requires that the authors and source are credited.
The license is subject to the Beilstein Archives terms and conditions: (https://www.beilstein-archives.org/xiv/terms)

Other Beilstein-Institut Open Science Activities

Logo
Logo
Logo
Symposia