Dual-Functionalized Architecture Enables Stable and Tumor Cell-Specific SiO2NPs in Complex Biological Fluids

Submitting author affiliation:
LNLS, Campinas, Brazil

Beilstein Arch. 2024, 202442. https://doi.org/10.3762/bxiv.2024.42.v1

Published 19 Jun 2024

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This preprint has not been peer-reviewed. When a peer-reviewed version is available, this information will be updated.


Most commercial anti-cancer nanomedicines are administered intravenously. This route is fast and precise as the drug enters directly into the systemic circulation, without undergoing absorption processes. When nanoparticles come into direct contact with the blood, however, they interact with physiological components that can induce colloidal destabilization and/or changes in their original biochemical identity, compromising their ability to selectively accumulate at target sites. In this way, these systems usually lack active targeting, offering limited therapeutic effectiveness. In the literature, there is a paucity of in-depth studies in complex environments to evaluate nanoparticle stability, protein corona formation, hemolytic activity, and targeting capabilities. To address this issue, fluorescent silica nanoparticles (SiO2NPs) are here functionalized with zwitterionic (kinetic stabilizer) and folate groups (targeting agent) to provide selective interaction with tumor cell lines in biological media. The stability of these dually functionalized SiO2NPs is preserved in unprocessed human plasma while yielding a decrease in the number of adsorbed proteins. Experiments in rat blood further proved that these nanoparticles are not hemolytic. Remarkably, the functionalized SiO2NPs are more internalized by tumor cells than their healthy counterparts. Investigations of this nature play a crucial role in garnering results with greater reliability, allowing the development of nanoparticle-based pharmaceutical drugs that exhibit heightened efficacy and reduced toxicity for medical purposes.

Keywords: functionalized nanoparticles; complex media; colloidal stability; hemolysis; targeting tumor

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When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Sousa Ribeiro, I. R.; da Silva, R. F.; Domingues, R. R.; Leme, A. F. P.; Cardoso, M. B. Beilstein Arch. 2024, 202442. doi:10.3762/bxiv.2024.42.v1

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