Beilstein Arch. 2023, 20231. https://doi.org/10.3762/bxiv.2023.1.v1
Published 02 Jan 2023
Two novel hybrid molecules were designed: pyrene and phenanthridine-amino acid units with a different linker length between aromatic fragments. Molecular modelling combined with spectrophotometric experiments revealed that in neutral and acidic buffered water solutions conjugates predominantly exist in intramolecularly stacked conformations as a result of the π-π stacking interaction between pyrene and phenanthridine moieties. Investigated systems exhibited pH-dependent excimer formation that is significantly red-shifted relative to the pyrene and phenanthridine fluorescence. While conjugate with a short linker showed negligible spectrophotometric changes due to the polynucleotide addition, conjugate with longer and more flexible linker exhibited a micro-molar and sub-micro-molar binding affinity for ds-polynucleotides and inactive mutant of dipeptidyl peptidase enzyme E451A.
Keywords: dipeptidyl peptidase enzyme; excimer; phenanthridine; polynucleotide; pyrene
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Matić, J.; Tandarić, T.; Radić Stojković, M.; Šupljika, F.; Vianello, R.; Tumir, L.-M. Beilstein Arch. 2023, 20231. doi:10.3762/bxiv.2023.1.v1
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