Beilstein Arch. 2022, 202262. https://doi.org/10.3762/bxiv.2022.62.v1
Published 13 Jul 2022
In this study the antimicrobial effect of silver nanoparticles (AgNPs) synthesized from Coriandrum sativum (C. sativum) leaf extract was evaluated by varying the incubation temperature from 25 °C to 40 °C, for 24 h of exposure. AgNPs were characterized using Ultraviolet-Visible (UV-vis), and Transmission Electron (TEM) spectroscopy, evidencing the presence of nanoparticles of spherical morphology with average sizes of 5.7, 13.4, and 6.0 nm for nanoparticle concentrations of 1, 10, and 100 mM, respectively. The efficacy of nanoparticles as microbicidal agents was evaluated with the halo inhibition method, impregnating nanoparticles in sensi-discs on agar planted with ATCC standard Staphylococcus aureus (S. aureus), and clinically isolated Klebsiella pneumoniae (K. pneumoniae) and Pseudomonas aeruginosa (P. aeruginosa). AgNPs inhibited the growth of all three bacteria under all conditions. The clinically isolated bacteria obtained the smallest inhibitory diameters, with respect to the standard bacteria. Incubation temperature had a significant effect on all bacteria, with the greatest effect found with AgNPs-100mM at 25°C for P. aeruginosa and at 35°C for S. aureus and K. pneumoniae. These results showed the potential application against pathogenic microorganisms of AgNPs synthesized from C. sativum leaf in hospital environments within a temperature range from 25 to 40 °C.
Keywords: Silver nanoparticles; antimicrobial effect; S. aureus; K. pneumoniae; P. aeruginosa; C. sativum; hospital environments
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Barraza-Vergara, L. F.; Llinás-Giraldo, M. A.; Albis Arrieta, A. R.; Medina Buelvas, A. M.; Barros Martínez, A. Beilstein Arch. 2022, 202262. doi:10.3762/bxiv.2022.62.v1
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