Characterization of a new fusicoccane-type diterpene synthase and an associated P450 enzyme

Submitting author affiliation:
Jinan University, Guangzhou, China

Beilstein Arch. 2022, 202247.

Published 09 Jun 2022

This preprint has not been peer-reviewed. When a peer-reviewed version is available, this information will be updated.


Fusicoccane-type terpenoids are a subgroup of diterpenoids featured with a unique 5-8-5 ring system. They are widely distributed in nature and possess a variety of biological activities. Up to date, only five fusicoccane-type diterpene synthases have been identified. Here, we identify a two-gene biosynthetic gene cluster containing a new fussicoccane-type diterpene synthase gene tadA and an associated cytochrome P450 gene tadB from Talaromyces wortmannii ATCC 26942. Heterologous expression reveals that TadA catalyzes the formation of a new fusicoccane-type diterpene talaro-7,13-diene. D2O isotope labeling combined with site-directed mutagenesis indicate that TadA might employ a different C2,6 cyclization strategy from the known fussicoccane-type diterpene synthases, in which a neutral intermediate is firstly formed and then protonated by an environmental proton. In addition, we demonstrate that the associated cytochrome P450 enzyme TadB is able to catalyze multiple oxidation of talaro-7,13-diene to yield talaro-6,13-dien-5,8-dione.

Keywords: genome mining; diterpene synthase; gene cluster; site-directed mutagenesis; cytochrome P450 enzyme

Supporting Information

Format: DOCX Size: 13.7 MB   Download

How to Cite

When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Huang, J.-H.; Lv, J.-M.; Xiao, L.-Y.; Xu, Q.; Lin, F.-l.; Wang, G.-Q.; Chen, G.-D.; Qin, S.-Y.; Hu, D.; Gao, H. Beilstein Arch. 2022, 202247. doi:10.3762/bxiv.2022.47.v1

Download citation
Download RIS (Reference Manager) Download BIB (BIBTEX)

© 2022 Huang et al.; licensee Beilstein-Institut.
This is an open access work licensed under the terms of the Beilstein-Institut Open Access License Agreement (, which is identical to the Creative Commons Attribution 4.0 International License ( The reuse of material under this license requires that the author(s), source and license are credited. Third-party material in this work could be subject to other licenses (typically indicated in the credit line), and in this case, users are required to obtain permission from the license holder to reuse the material.