Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation

Sergey A. Staroverov; Sergey V. Kozlov; Alexander S. Fomin; Daniil S. Chumakov; Konstantin P. Gabalov; Yevgeny S. Kozlov; Dmitry A. Soldatov; Lev A. Dykman

doi:10.3762/bxiv.2022.28.v1

Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation

Sergey A. Staroverov ,
Sergey V. Kozlov ,
Alexander S. Fomin ,
Daniil S. Chumakov ,
Konstantin P. Gabalov ,
Yevgeny S. Kozlov ,
Dmitry A. Soldatov and
Lev A. Dykman

Submitting author affiliation:

Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Saratov, Russian Federation

Beilstein Arch. 2022, 202228. https://doi.org/10.3762/bxiv.2022.28.v1

Published 26 Apr 2022

Preprint

PDF

This preprint has not been peer-reviewed. When a peer-reviewed version is available, this information will be updated.

SUBJECT AREAS

Show All Preprints Nanotechnology Organic chemistry Bioorganic chemistry / chemical biology Catalysis Chemical methods and reactions Flow and process chemistry Green and sustainable chemistry Materials chemistry Medicinal and pharmaceutical chemistry Nano- and molecular-scale electronics Nano-biomaterials and bioscience Nanomagnetics Nanomaterials, thin films and nanointerfaces Nanomedicine Nanometrology and nanomechanics Nano-optics Nanopatterning, self-assembly and nanofabrication Nanostructures for energy and sensing applications Natural products chemistry Organo main group chemistry Other nanotechnology (unclassified) Other organic chemistry (unclassified) Photochemistry and photovoltaics Physical organic chemistry Supramolecular chemistry

Abstract

Gold nanoparticles as part of vaccines greatly increase antigen stability, antigen accumulation in the lymph nodes, and antigen uptake by antigen-presenting cells. The use of such particles as part of anticancer vaccines based on heat shock proteins to increase vaccine effectiveness is timely. We prepared and characterized nanoconjugates based on 15-nm gold nanoparticles and thermostable tumor antigens isolated from MH22a murine hepatoma cells. BALB/c mice were injected with the conjugates and then received transplants of MH22a cells. The immunized mice showed no signs of tumor growth for 24 days. They also showed decreased production of the INF-γ, IL-6, and IL-1 proinflammatory cytokines, as compared to the mice immunized through other schemes. This study is the first to show that it is possible in principle to use gold nanoparticles in combination with thermostable tumor antigens for antitumor vaccination. Antitumor vaccines based on thermostable tumor antigens can be largely improved by including gold nanoparticles as additional adjuvants.

Keywords: Gold nanoparticles; heat shock proteins; vaccination; adjuvant; tumor transplantation

How to Cite

When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Staroverov, S. A.; Kozlov, S. V.; Fomin, A. S.; Chumakov, D. S.; Gabalov, K. P.; Kozlov, Y. S.; Soldatov, D. A.; Dykman, L. A. Beilstein Arch. 2022, 202228. doi:10.3762/bxiv.2022.28.v1

Download Citation

Citation data can be downloaded as file using the "Download" button or used for copy/paste from the text window below.
Citation data in RIS format can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Zotero.

File format:

RIS

BibTeX

Download

© 2022 Staroverov et al.; licensee Beilstein-Institut.
This is an open access work licensed under the terms of the Beilstein-Institut Open Access License Agreement (https://www.beilstein-archives.org/xiv/terms), which is identical to the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0). The reuse of material under this license requires that the author(s), source and license are credited. Third-party material in this work could be subject to other licenses (typically indicated in the credit line), and in this case, users are required to obtain permission from the license holder to reuse the material.

aromatic	the word “aromatic”
aromatic aldehyde	the word “aromatic” OR “aldehyde”
+aromatic +aldehyde	both words “aromatic” AND “aldehyde”
+aromatic -aldehyde	the word “aromatic” but NOT “aldehyde”
“aromatic aldehyde”	the exact phrase “aromatic aldehyde”
benz*	words which begin with “benz”, such as “benzene” or “benzyl”
benz*yl	words that begin with “benz” and end with “yl”, such as “benzyl” or “benzoyl”
benzyl~	words that are close to the word “benzyl”, such as “benzoyl” (i.e., fuzzy search)