Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation

Submitting author affiliation:
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Saratov, Russian Federation

Beilstein Arch. 2022, 202228.

Published 26 Apr 2022

This preprint has not been peer-reviewed. When a peer-reviewed version is available, this information will be updated.


Gold nanoparticles as part of vaccines greatly increase antigen stability, antigen accumulation in the lymph nodes, and antigen uptake by antigen-presenting cells. The use of such particles as part of anticancer vaccines based on heat shock proteins to increase vaccine effectiveness is timely. We prepared and characterized nanoconjugates based on 15-nm gold nanoparticles and thermostable tumor antigens isolated from MH22a murine hepatoma cells. BALB/c mice were injected with the conjugates and then received transplants of MH22a cells. The immunized mice showed no signs of tumor growth for 24 days. They also showed decreased production of the INF-γ, IL-6, and IL-1 proinflammatory cytokines, as compared to the mice immunized through other schemes. This study is the first to show that it is possible in principle to use gold nanoparticles in combination with thermostable tumor antigens for antitumor vaccination. Antitumor vaccines based on thermostable tumor antigens can be largely improved by including gold nanoparticles as additional adjuvants.

Keywords: Gold nanoparticles; heat shock proteins; vaccination; adjuvant; tumor transplantation

How to Cite

When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Staroverov, S. A.; Kozlov, S. V.; Fomin, A. S.; Chumakov, D. S.; Gabalov, K. P.; Kozlov, Y. S.; Soldatov, D. A.; Dykman, L. A. Beilstein Arch. 2022, 202228. doi:10.3762/bxiv.2022.28.v1

Download citation
Download RIS (Reference Manager) Download BIB (BIBTEX)

© 2022 Staroverov et al.; licensee Beilstein-Institut.
This is an open access work licensed under the terms of the Beilstein-Institut Open Access License Agreement (, which is identical to the Creative Commons Attribution 4.0 International License ( The reuse of material under this license requires that the author(s), source and license are credited. Third-party material in this work could be subject to other licenses (typically indicated in the credit line), and in this case, users are required to obtain permission from the license holder to reuse the material.