Functional nanomaterials with simulated properties have become promising candidates for the detection of hydrogen peroxide. However, there are few studies on the colorimetric detection of metal ions and amino acids based on the peroxidase-simulation activity of amino acid functionalized AuNCs. In this study, a method for preparing fluorescent probe using histidine (His) and ascorbic acid (AA) as reductant and stabilizer was proposed. TMB was used as chromogenic substrate to indicate the catalytic process. The ultraviolet absorbance of oxTMB was determined at the characteristic absorption peak 625nm, and the standard curve was drawn to determine the concentration of H2O2 accurately. We found that Fe3+ can greatly improve the response signal of His-AA-AuNCs and has high selectivity. The linear range of H2O2 concentration detection is 10-9.97×106 μM. The concentration range of probe response to Fe3+ is 0.28-280 nM. The His-AA-AuNCs fluorescent probe was applied to intracellular fluorescence imaging after adriamycin injury, and the fluorescence intensity increased with the increase of probe concentration. This study was based on the double-stranded nature of amino acids and the properties of hydrogen peroxide mimic enzymes to detect other substances, which has a promising application prospect, and may potentially be applied to metal ions, amino acids and peptides in the biological and environmental fields in the future.
Keywords: Cell imaging; Colorimetry; Gold nanoclusters; Iron ions; Oxidative mimetic enzymes.
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Li, S.; Cai, J.; Zhang, Y.; Shi, P.; Chen, B.; Huang, L.; Lin, Z.; Lin, X.; Yao, H. Beilstein Arch. 2020, 202032. doi:10.3762/bxiv.2020.32.v1
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