Colorimetric detection of organophosphates with cysteamine capped gold nanoparticle sensors

Submitting author affiliation:
University of Illinois at Urbana-Champaign, Urbana, United States

Beilstein Arch. 2020, 2020137.

Published 04 Dec 2020

cc-by Logo
This preprint has not been peer-reviewed. When a peer-reviewed version is available, this information will be updated.


Nanozyme biosensors have the potential to provide high sensitivity, multiple functionality, and tunable activity. A facile colorimetric biosensor for the detection of organophosphates (OPs) using cysteamine capped gold nanoparticle probes (C-AuNPs) as enzyme mimics is proposed. Parathion ethyl (PE) a class of OPs is a potent insecticide that functions by inhibiting the acetylcholinesterase (AChE) in the nervous system of insects. The inhibition kinetics of AChE using PE enables the development of a PE sensor. C-AuNPs possess the ability to catalyze the oxidization of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) to a blue-colored product without peroxidase. The detection of PE was monitored by the inability of AChE to generate choline. Choline causes the aggregation of C-AuNPs and the aggregated C-AuNPs has decreased ability to catalyze the oxidization of TMB. A calibration was developed in the 40-320 nM range for the quantitative detection of PE. The limit of detection observed was 20 nM and the method had excellent specificity. The proposed sensor provides an excellent platform for on-site monitoring of PE in environmental and food samples with high sensitivity and greater selectivity.

Keywords: Colorimetric biosensor; Cysteamine capped gold nanoparticles; Nanozyme; Neurotoxin; Parathion ethyl

Supporting Information

Format: DOCX Size: 266.4 KB   Download

Previous Versions

Format: PDF Size: 919.1 KB Version: 1 Download

How to Cite

When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Shah, M. M.; Ren, W.; Ahmad, B.; Irudayaraj, J. Beilstein Arch. 2020, 2020137. doi:10.3762/bxiv.2020.137.v1

Download Citation

Citation data can be downloaded as file using the "Download" button or used for copy/paste from the text window below.
Citation data in RIS format can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Zotero.