Beilstein J. Org. Chem.2006,2, No. 13, doi:10.1186/1860-5397-2-13
Laboratories (San Francisco, CA), was diluted 1:100. As secondary antibodies, FITC-conjugated (U. S. Biochemical Corp., Cleveland, OH) or Alexa 488-conjugated goat anti-mouse immunoglobulin G (Molecular Probes, Eugene, OR) were used as described previously [17]. Wide-field fluorescence images were recorded on
PDF
Graphical Abstract
Scheme 1:
Reagents and conditions for synthesis of N-glutaryl-deacetylcolchicine. The reagents used at each s...